Anchorchip manual

When we use anchorchip, sample spotting should be done in different way than normal. Sample spotting on anchorchip: (p15 of anchorchip manual) prepare following reagents. matrix solution: HCCA(alpha-cyanohydroxy cinnamic acid) mg/ml in ACN 1 ml vortex, centrifuge, and use supernatant. Document History ClinProTools User Manual, Version (November ) Part #: First edition: June Printed in Germany Warranty The information contained in this document is subject to change without notice. Bruker Daltonik GmbH makes no warranty of any kind with regard to this material, including, but not limited to, the implied Missing: anchorchip. Search for PDF and other binary Assets Please use at least 2 characters (you are currently using 1 character).

Whereas disposable Prespotted AnchorChip (PAC) targets (# and #) do not require a washing / cleaning procedure prior to sample preparation, reusable targets require appropriate cleaning for optimal performance. For washing and cleaning of steel AnchorChip Targets, please follow the procedure described in the AnchorChip manual. Preparation Protocols manual. Matrix Sample Type Sample Solvent Matrix Solubilization Sample Preparation. HCCA Peptides, protein digests TA30 solvent or % TFA Saturated solution of HCCA in TA30 - Mix 1 part Matrix with 1 part sample solution - Deposit µL of mixture onto target and allow to dry 2,5-DHB Peptides, phosphoprotein. AnchorChip targets are scout MTP MALDI targets equipped with a special coating, which provides improved crystallization of MALDI samples with the following benefitsfold sensitivity increasedue to increased analyte concentration Improved automationLess hunting for sweet spots, as samples are homogeneous and sit on predefined.

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